Successfully complete the Ghetto level with Donatello to unlock April's adventure mode.

Successfully complete the Subway level with Raphael to unlock Casey Jones' adventure mode.

Successfully complete Raphael's adventure mode to unlock Foot Soldier's adventure mode.

Successfully complete Michaelangelo's adventure mode to unlock HUN's adventure mode.

Successfully complete Donatello's adventure mode to unlock Shredder's adventure mode.

Successfully complete Leonardo's adventure mode to unlock Splinter's adventure mode.

Successfully complete Casey Jones' adventure mode with April to unlock Casey Jones' jacket costume.

Successfully complete the Ghetto level with Casey Jones to unlock Casey Jones' red shorts costume.

Successfully complete Shredder's adventure mode to unlock Shredder's gold costume.

Successfully complete the April's Apartment 2 level with Donatello to unlock April's Apartment stage.

Successfully complete The Dojo level with Michaelangelo to unlock the Autumn stage.

Successfully complete the Turtle's Lair level with Casey Jones to unlock the Dojo Complete stage.

Successfully complete the Sewer Falls level with Raphael to unlock the Sewer Falls stage.

Successfully complete the Sewers level with Leonardo to unlock the Sewers stage.

Successfully complete The Lab 12 level with Leonardo to unlock the Shredder's Palace Exterior stage.

Successfully complete the Sparring With Splinter level with Leonardo to unlock the Subway stage.

Successfully complete the Turtle's Lair level with April to unlock the Waterfall stage.