Get a "Gold" rank on all regular Tournament courses to unlock the Pro Tournament.

Win all the Pro Tournament courses to unlock the Mirror Tournament.

Win on a course in Single Player Tournament mode to unlock that course.

Defeat a CPU character in Single Player Vs. Match mode to unlock that person as a playable character. Unlock all CPU characters to also unlock your Mii as a playable character in all Single Player modes.

Successfully complete the indicated task to unlock the corresponding costume:

Apollo Justice (Apollo Justice: Ace Attorney) costume for Jack: Win on all courses in Tournament mode.
Arthur (Ghosts N' Goblins) costume for Carlos: Successfully complete all Ring Challenges.
Captain Rose (Zack And Wiki: Quest For Barbaros' Treasure) costume for Marissa: Get a "Gold" rank on all Pro Tournament courses.
Chun-Li (Street Fighter) costume for Lisa: Successfully complete Second Shot on all three difficulties.
Guile (Street Fighter) costume for Brian: Get a "Gold" rank on all Mirror Tournament courses.
Jill Valentine (Resident Evil) costume for Yuki: Successfully complete Putting on all three difficulties.
Ken (Street Fighter) costume for Mark: Win a Friend match.
Morrigan (Darkstalkers) costume for Stephanie: Win a World Play match in Wi-Fi mode.
Pearl Fey (Phoenix Wright) costume for Annie: Successfully complete Tee Shot on all three difficulties.
Ryu (Street Fighter) costume for Tony: Successfully complete Approach Shots on all three difficulties.
Sakura (Street Fighter 2) costume for Meg: Successfully complete all the Wii-mote training missions.
Zack (Zack And Wiki: Quest For Barbaros' Treasure) costume for Leo: Hit all the greens in the Near Pin Contest on all 18 hole courses.